SAS Data Analysis
In a good quality SAS experiment, several solutions with varying concentrations of the macromolecule under investigation are measured. By extrapolating the scattering curves measured at different concentrations to zero concentration, one is able to obtain a scattering curve that represents infinite dilution. Then concentration effects should not affect the scattering curve. Data analysis of the extrapolated scattering curve begins with the inspection of the start of the scattering curve in the region around q = 0. If the region follows the Guinier approximation (also known as Guinier law), the sample is not aggregated. Then the shape of the particle in question can be determined by various methods, of which some are described in the following reference.
Read more about this topic: Biological Small-angle Scattering
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