Biernacki (feminine Biernacka) is a Polish surname, it may refer to:
- Edmund Biernacki, Polish physician
- Marek Biernacki, Polish politician
- Marianna Biernacka, Polish martyr of World War II
- Mieczysław Biernacki, Polish mathematical chemist
- Stefan Dąb-Biernacki, Polish general
- Wacław Kostek-Biernacki, Polish politician and writer
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