BBO may stand for:
- Big Bang Observer, planned space gravitational wave observatory
- Beta barium borate (β-BaB2O4) crystal
- Black Bag Operations
- Bureau Bijzondere Opdrachten, a Dutch secret service during World War II
- The Bridge Base Inc. web site Bridge Base Online
- British Ballet Organisation, a dance teaching and examination board
- Broome Bird Observatory
- BBO, the ICAO code for Flybaboo
- Bottom Bottle Opener, a cap-shaped indentation found in the bottom of Miller High Life bottles in the late 1980s which could be used to open other bottles of Miller High Life.
- Best Bid Offer, a type of financial quote that includes the best bid and ask (offer) prices
- Biogeography-Based Optimization, a type of evolutionary algorithm