Apoptotic DNA Fragmentation

Apoptotic DNA fragmentation is a key feature of apoptosis, a type of programmed cell death. Apoptosis is characterized by the activation of endogenous endonucleases with subsequent cleavage of chromatin DNA into internucleosomal fragments of roughly 180 base pairs (bp) and multiples thereof (360, 540 etc.). This effect can be used to detect apoptosis, for example via the DNA laddering assay, the TUNEL assay, or the Nicoletti assay.

Read more about Apoptotic DNA Fragmentation:  Mechanism, Historical Background, Detection Assays

Other articles related to "dna, apoptotic dna fragmentation, dna fragmentation":

Royal Jelly - Epigenetic Effects
... In spite of their identical clonal nature at the DNA level, they are strongly differentiated across a wide range of characteristics including anatomical and physiological ... It has been shown that this phenomenon is mediated by an epigenetic modification of DNA known as CpG methylation ... Silencing the expression of an enzyme that methylates DNA in newly hatched larvae led to a royal jelly-like effect on the larval developmental trajectory the majority of ...
Apoptotic DNA Fragmentation - Detection Assays
... There are many methods to assess the DNA fragmentation caused by apoptosis and also many commercial kits are available ... Some new methods do quantification assays for DNA Fragmentation by flow cytometry and fluorescent assays ... Apoptotic DNA fragmentation is often analyzed using agarose gel electrophoresis to demonstrate a "ladder" pattern at ~180-BP intervals ...
Filter Binding Assay
... It measures affinities between two molecules (often protein and DNA) using a filter ... DNA is negatively charged due to the phosphate backbone and will not "stick" to the nitrocellulose on its own, however, any DNA that has been bound by protein will stick ... The exact amount of DNA "stuck" to the nitrocellulose is quantified by measuring the amount of radioactivity on the filter using a scintillation counter Protein ...
History of DNA Research
... Further information History of molecular biology DNA was first isolated by the Swiss physician Friedrich Miescher who, in 1869, discovered a microscopic substance in the pus of discarded surgical bandages ... Levene suggested that DNA consisted of a string of nucleotide units linked together through the phosphate groups ... diffraction patterns that showed that DNA had a regular structure ...
DNA Lounge - History
... by Jim English, Jeff Mason, and Brian Raffi who opened for business under the name DNA Lounge on November 22, 1985 ... Many of DNA's posters and flyers were designed by the San Francisco-based artist, Rex Ray ... During the early 90s, the DNA offered a black membership card for $10 that offered free entry most nights ...

Famous quotes containing the word dna:

    Here [in London, history] ... seemed the very fabric of things, as if the city were a single growth of stone and brick, uncounted strata of message and meaning, age upon age, generated over the centuries to the dictates of some now all-but-unreadable DNA of commerce and empire.
    William Gibson (b. 1948)