Antimicrobial Peptides - Mode of Action

Mode of Action

Several methods have been used to determine the mechanisms of antimicrobial peptide activity. In particular, solid-state NMR studies have provided an atomic-level resolution explanation of membrane disruption by antimicrobial peptides.

Methods Applications
Microscopy to visualize the effects of antimicrobial peptides on microbial cells.
Fluorescent dyes to measure antimicrobial peptides to permeabilize membrane vesicles.
Ion channel formation to assess the formation and stability of an antimicrobial-peptide-induced pore.
Circular dichroism and orientated circular dichroism to measure the orientation and secondary structure of an antimicrobial peptide bound to a lipid bilayer
Dual Polarization Interferometry to measure the different mechanisms of antimocrobial peptides
Solid-state NMR spectroscopy to measure the secondary structure, orientation and penetration of antimicrobial peptides into lipid bilayers in the biologically relevant LIQUID-CRYSTALLINE STATE
Neutron and X-ray diffraction to measure the diffraction patterns of peptide-induced pores within membranes in oriented multilayers or liquids

Read more about this topic:  Antimicrobial Peptides

Famous quotes containing the words mode of, mode and/or action:

    Happiness is a matter of one’s most ordinary everyday mode of consciousness being busy and lively and unconcerned with self. To be damned is for one’s ordinary everyday mode of consciousness to be unremitting agonising preoccupation with self.
    Iris Murdoch (b. 1919)

    I have no scheme about it,—no designs on men at all; and, if I had, my mode would be to tempt them with the fruit, and not with the manure. To what end do I lead a simple life at all, pray? That I may teach others to simplify their lives?—and so all our lives be simplified merely, like an algebraic formula? Or not, rather, that I may make use of the ground I have cleared, to live more worthily and profitably?
    Henry David Thoreau (1817–1862)

    The highest proof of civility is that the whole public action of the State is directed on securing the greatest good of the greatest number.
    Ralph Waldo Emerson (1803–1882)