Anna Anderson - DNA Evidence

DNA Evidence

In 1991, the bodies of Tsar Nicholas II, Tsarina Alexandra, and three of their daughters were exhumed from a mass grave near Ekaterinburg. They were identified on the basis of both skeletal analysis and DNA testing. For example, mitochondrial DNA was used to match maternal relations, and mitochondrial DNA from the female bones matched that of Prince Philip, Duke of Edinburgh, whose maternal grandmother Princess Victoria of Hesse and by Rhine was a sister of Alexandra. The bodies of Tsarevich Alexei and the remaining daughter were discovered in 2007. Repeated and independent DNA tests confirmed that the remains were the seven members of the Romanov family, and proved that none of the Tsar's four daughters survived the shooting of the Romanov family.

A sample of Anderson's tissue, part of her intestine removed during her operation in 1979, had been stored at Martha Jefferson Hospital, Charlottesville, Virginia. Anderson's mitochondrial DNA was extracted from the sample and compared with that of the Romanovs and their relatives. It did not match that of the Duke of Edinburgh or that of the bones, confirming that Anderson was not Anastasia. However, the sample matched DNA provided by Karl Maucher, a grandson of Franziska Schanzkowska's sister, Gertrude (Schanzkowska) Ellerik, indicating that Karl Maucher and Anna Anderson were maternally related and that Anderson was Schanzkowska. Five years after the original testing was done, Dr. Terry Melton of the Department of Anthropology, Pennsylvania State University, stated that the DNA sequence tying Anderson to the Schanzkowski family was "still unique", though the database of DNA patterns at the Armed Forces DNA Identification Laboratory had grown much larger, leading to "increased confidence that Anderson was indeed Franziska Schanzkowska".

Similarly, several strands of Anderson's hair, found inside an envelope in a book that had belonged to Anderson's husband, Jack Manahan, were also tested. Mitochondrial DNA from the hair matched Anderson's hospital sample and that of Schanzkowska's relative Karl Maucher but not the Romanov remains or living relatives of the Romanovs.