Isolation
As stated previously, these alkaloids were first discovered in the skins of frogs. They can be isolated from the frog’s skin by mincing the skin and extracting the compounds by trituration. A series of extractions involving an acid-base extraction are needed to isolate the allopumiliotoxins. The skins of the frogs may contain a number of different allopumiliotoxins. For example, the skin of Dendrobates tricolor was found to contain alkalkoids 251D, 271, 341A, and 323B. Also, different frogs contain different alkaloids in their skin. Dendrobates auratus, for example, was found to contain (+)-allopumiliotoxin 339A (a compound not present in the skin of Dendrobates tricolor).
Read more about this topic: Allopumiliotoxin
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