An alewife is an archaic English word for a woman who keeps an inn or tavern where ale is brewed and served. Alewife may also refer to:
- Alewife, a kind of fish
- Alewife Brook Reservation, a state park in Massachusetts
- Alewife (MBTA station), a local transportation hub in Cambridge, Massachusetts
- Alewife (multiprocessor), a computer system developed at the Massachusetts Institute of Technology
- Dead Alewives, an improvisational comedy troupe