Alanine Dehydrogenase

Alanine dehydrogenase (EC 1.4.1.1) is an enzyme that catalyzes the chemical reaction

L-alanine + H₂O + NAD+ pyruvate + NH₃ + NADH + H+

The 3 substrates of this enzyme are L-alanine, water, and nicotinamide adenine dinucleotide+, whereas its 4 products are pyruvate, ammonia, NADH, and hydrogen ion.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is L-alanine:NAD+ oxidoreductase (deaminating). Other names in common use include AlaDH, L-alanine dehydrogenase, NAD+-linked alanine dehydrogenase, alpha-alanine dehydrogenase, NAD+-dependent alanine dehydrogenase, alanine oxidoreductase, and NADH-dependent alanine dehydrogenase. This enzyme participates in taurine and hypotaurine metabolism and reductive carboxylate cycle (CO₂ fixation).