Agnolo Gaddi (born c. 1350 in Florence; active 1369–1396; died 1396 in Florence) was an Italian painter. He was the son of the painter Taddeo Gaddi.
Taddeo Gaddi was himself the major pupil of the Florentine master Giotto. Agnolo was an influential and prolific artist who was the last major Florentine painter stylistically descended from Giotto.
Giorgio Vasari included a biography of Agnolo Gaddi in his Lives of the Most Excellent Painters, Sculptors, and Architects. Gaddi's work includes frescoes in the choir of Santa Croce in Florence as well as:
- Madonna Enthroned with Saints and Angels (1380/1390), tempera on panel. Triptych, possibly commissioned for a Cistercian monastery.
- The Coronation of the Virgin (c.1380-90), tempera on wood. This painting is probably the central panel of an altarpiece, possibly from the church of San Giovanni de' Fieri near Pisa.
- Madonna of Humility with Angels (mid 1390s), tempera on panel
- Crucifixion (no date), tempera on wood. Part of a polyptych.
- Histories of the Virgin and the Cintola in Prato Cathedral