Isolation
The venom of the Agelenopsis aperta is located in two glands, which are located in the two fang bases. Ejection of the venom takes place via contraction of surrounding muscles. To obtain this venom, the spider is milked by electrical stimulation. The crude venom is dissolved in an EDTA plasma to avoid proteolysis. Purification of the agatoxin is accomplished by a HPLC procedure.
Read more about this topic: Agatoxin
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